Adenosine deaminase from calf intestinal mucosa.
نویسنده
چکیده
In the course of studying the phosphoric acid esterases from calf intestinal mucosa (1, 2) the preparations were found to contain a potent deaminase for adenosine. The method of preparation is simple and the procedure indicates that this enzyme is quite stable. This deaminase is probably identical with that recently prepared by Brady (3) from the same material by a somewhat different procedure. In the present studies the action of the enzyme was followed manometrically ; the utilization of this procedure for the estimation of adenosine is suggested.
منابع مشابه
EFFECT OF BORATE ON A PROTEIN-POLYSACCHARIDE COMPLEX, THE PHOSPHOESTERASE FROM CALF INTESTINAL MUCOSA sirs: An enzyme preparation from calf intestinal mucosa
An enzyme preparation from calf intestinal mucosa was studied for phosphodiesterase activity’ but was found to contain also phosphomonoesterase’ and adenosine deaminase.2 Analysis confirmed the presence of polysaccharide, suspected because of solubility in high concentrations of organic solvents and precipitation from aqueous solutions only when fully saturated with ammonium sulfate. Attempts w...
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In fat cells isolated from the parametrial adipose tissue of rats, the addition of purified adenosine deaminase increased lipolysis and cyclic adenosine 3’: 5’-monophosphate (cyclic AMP) accumulation. Adenosine deaminase markedly potentiated cyclic AMP accumulation due to norepinephrine. The increase in cyclic AMP due to adenosine deaminase was as rapid as that of theophylline with near maximal...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 166 2 شماره
صفحات -
تاریخ انتشار 1946